In wild-type animals, the PSA-3::GFP expression in the T.p cell was higher than (
log2(p3Tp/p3Ta) >=0.5) or almost equal (0.5 >
log2(p3Tp/p3Ta)>=-0.5) to that in the T.a cell. No T.p cell expressed lower (
log2(p3Tp/p3Ta)<-0.5) PSA-3::GFP than the T.a cells. Although in the
pbrm-1 and
let-526 mutant, 1 of 21 and 2 of 19 T.p cells, respectively, expressed lower PSA-3::GFP than the T.a cell, this difference was not statistically significant. In the
let-526 pbrm-1 double mutant, significant numbers of an- imals had T.p cells with less PSA-3::GFP expression than their T.a sister (p = 0.02). When we statistically analyzed the distribution of all the
log2(p3Tp/p3Ta) values by a t test, the
let-526 pbrm-1 double mutant (p=0.04), but not the
let-526 (p=0.52) or
pbrm-1 (p = 0.36) single mutant, was significantly different from the wild- type animals.