"Similarly, a GFP reporter construct of the multi-TM protein CHE-14 (Michaux et al., 2000) was used to mark the apical surface of a subset of epidermal cells (Fig. 3G). We observed that these proteins all remained at the apical surface in
dlg-1(RNAi) embryos (Fig. 3B,B',E,E',H), although PAR-3 appeared to move to a position coincident with, or just apical to, the CeAJ (marked by HMP-1) in the intestine (Fig. 3B'), and small areas of subapical localisation were also detected for CHE-14::GFP in the epidermis (arrows in Fig. 3H). In contrast, PAR-3 and PAR-6 became progressively mislocalized basally along the lateral membrane of intestinal cells in
let-413(RNAi) embryos (Fig. 3C,F,C',F'). Similarly, CHE-14::GFP was progressively mislocalizedto the lateral cell membrane in LET- 413-deficient embryos (Fig. 3I)."