"To examine the role of
pdp-1 in regulating fat storage, we used both Oil Red O [45] and Sudan Black [7] staining (Figure 2F and 2G and Figure S7).
pdp-1 mutants had similar levels of fat compared to wild-type worms, while overexpression of
pdp-1 slightly enhanced fat storage (Figure S7). In contrast, a
pdp-1 mutation drastically reduced the increased fat of
daf-2(
e1370) mutants (Figure 2F and 2G and Figure S7). This was observed in dauers, larval stage 3 (L3) animals and adults, suggesting that PDP-1 is an important regulator of fat storage in
daf-2 mutants."