In the absence of IR, a specific band at 70 kDa was detected in the wild-type and
akt-1 mutants, whereas no band was detected in
cep-1(
gk138) deletion mutants. After IR treatment, a slower migrating band that likely represents a posttranslationally modified form of CEP-1/p53 was detected. The levels of phosphorylated CEP-1 were lower in
akt-1(
mg144) than in wild-type worms at all doses of IR tested. In these mutants, the unmodified 70 kDa band was also significantly lower in abundance after treatment with 60 and 120 Gy IR, whereas in wild-type worms, the levels remained unchanged in response to IR.