NPH-1 was not localized to the transition zone in the nph-4 mutants. NPH-4 function is required for normal localization of NPH-1 to the transition zone, and the NPH proteins function as part of the same complex.
RT-PCR analysis of nph-1 showed a marked decrease in expression of both genes in the daf-19(m86) mutant background compared to that seen in the daf-19(+) background. nph-1::CFP expression was greatly diminished in the daf-19(m86) background compared with daf-19(+).
RT-PCR analysis of nph-4 showed a marked decrease in expression of both genes in the daf-19(m86) mutant background compared to that seen in the daf-19(+) background.