"When this reporter was introduced into
acs-3(
ft5) and
nhr-25(
ku217) mutants, we observed a marked increase in GFP fluorescence (Figure S2). Similarly,
nhr-25(RNAi) also caused strong expression of the
nlp-29p::gfp reporter (Figure S2). The microarray, qPCR, and
nlp-29p::gfp reporter data indicated that
acs-3(lf) and
nhr-25(rf) lead to expression of immune genes during growth on a standard lab diet of E. coli OP50, with strong expression already apparent in L4 stage larvae."