"To identify miRNAs that function in the pathogen response, we extended upon our identification of intestine-enriched miRNAs by identifying miRNAs that are enriched in the L4 intestine and miRNAs that show altered association with the intestinal miRISC following exposure to P. aeruginosa... When we compared the relative abundance of miRNAs in both the intestinal miRISCs of OP50- and PA14-fed worms we detected highly reproducible changes in the relative abundance of several miRNAs between the two populations of worms (Figure 6B). These data indicate that the pathogen response is accompanied by dynamic changes in miRNA activities within the intestinal miRISC."
"To identify miRNAs that function in the pathogen response, we extended upon our identification of intestine-enriched miRNAs by identifying miRNAs that are enriched in the L4 intestine and miRNAs that show altered association with the intestinal miRISC following exposure to P. aeruginosa... When we compared the relative abundance of miRNAs in both the intestinal miRISCs of OP50- and PA14-fed worms we detected highly reproducible changes in the relative abundance of several miRNAs between the two populations of worms (Figure 6B). These data indicate that the pathogen response is accompanied by dynamic changes in miRNA activities within the intestinal miRISC."
"We used miRDeep2 (Friedlander et al. 2012) using miRNA sequences downloaded from miRBase (Kozomara and Griffiths-Jones 2011) to quantify the levels of miRNAs in infected versus uninfected animals... Down-regulated miRNAs included the abundant miRNAs miR-71 and miR-80, which we confirmed via qPCR (Supplemental Fig. 6)."