"We fixed and stained wild-type and
icp-1(RNAi) embryos using anti-AIR-2 and antitubulin antibodies. During the meiotic cycles of wild-type embryos, AIR-2 was localized to meiotic chromosomes during meiotic metaphase (8/9 embryos). AIR-2 failed to localize to meiotic chromosomes in
icp-1(RNAi) embryos (0/10 embryos, Figure 5a)... in
icp-1(RNAi) embryos... discrete localization of AIR-2 was absent (0/3 embryos, Figure 5b)."