APL-1::GFP transgenic rescue strain was crossed to the hypomorphic mutant
unc-104(
e1265). Interestingly, rather than observing an accumulation of the GFP fluorescence in the cell body, which traditionally results from the reduction of UNC-104 mediated vesicle transport, a dramatic decrease in the fluorescence from
apl-1::gfp was found on the
unc-104(
e1265) background, as measured by fluorescence intensity in a set of three head inter-neurons that consistently expressed
apl-1. Additionally, APL-1 fluorescence was absent from the processes of the neurons, quantified from a specific dorsal process that is consistently visible on the N2 background. Western blotting from L4 worms also detects a drop in APL-1 protein expression on the
unc-104(
e1265) background . qRT-PCR comparing
apl-1 expression between N2 and unc- 104
(e1265) backgrounds showed no differences, suggesting that the loss of APL-1::GFP is occurring at the protein level.