GFP-tagged dynamin (DYN- 1::GFP) and the AP2 alpha-subunit (APT-4::GFP) were both localized to diffraction-limited puncta adjacent to presynaptic elements (labeled with mRFP::SNB-1), suggesting that these reporters are localized to perisynaptic endocytic zones. DYN-1 and APT-4 puncta intensities were significantly increased in
unc-57 mutants, indicating increased synaptic abundance when endophilin was absent.