In the intestine, TRA-1 is predominantly nuclear localized in both males and hermaphrodites. However, overall TRA-1 protein levels are significantly lower in wild-type male intestine cells. Intestinal TRA-1 levels are about 70% higher in
cul-2 mutant males than in wild-type males. The mean intensity levels of TRA-1 staining in the germ cell nuclei of
cul-2 mutant adult males and hermaphrodites are similar to each other but are approximately twice the level observed in wild-type hermaphrodites.