"we examined mRNA levels of a subset of these genes in
ain-2[reduction-of-function (rf)];
ain-1(lf) double mutants in which miRISC activity is strongly and specifically reduced (Zhang et al., 2007). We found a significant upregulation in eight of the nine AIN-2-associated,
dcr-1- and pathogen-responsive genes we tested (we refer to these genes as miRISC-regulated pathogen-response [MRPR] genes hereafter) (Figure 4B). As controls, we examined the expression of
ges-1 (an intestine-specific, pathogen nonresponsive gene), F08G5.6 (a pathogen-responsive gene encoding mRNAs that were not detected in the miRISC), and
sod-3 (a general stressinduced gene) and found no change in the expression of these genes in
ain-2(rf);
ain-1(lf) double mutants."