ins-33 transcripts are absent in the L1 and then accumulate in the L2 stage. In
lin-14(lf) L1 animals,
ins-33 mRNA accumulates precociously. In
lin-4(lf) L2 larvae, where LIN-14 levels are abnormally high,
ins-33 message is reduced compared to wild type. These results indicate that
ins-33 gene expression is developmentally regulated and that
lin-14 negatively regulates
ins-33 during the first larval stage.