In
unc-6(
ev400) single mutants, all ACs attached to the BM at the P6.p 1-cell stage prior to the invasion. The ACs later started to detach from the BM staying afloat in the gonad. The percentage of detached ACs increased to 10% and 15% at the P6.p 2- and 4-cell stages, respectively, suggesting that UNC-6 promotes AC-BM adhesion during AC invasion. In contrast,
mig-10(
ct41) mutants had no phenotypic defects in AC-BM attachment. However, when
mig-10 was removed in
unc-6 mutants, AC detachment was observed at the P6.p 1-cell stage, revealing an early requirement for MIG-10B in 215 stabilizing AC-BM attachment . AC detachment was later drastically increased by approximately 4-6 folds at the P6.p 2- and 4-cell stages, indicating a synergistic effect between MIG-10B and UNC-6 on mediating AC-BM adhesion during AC invasion.