"We also find that the level of clk-2 transcripts in fem-3(q20ts) adults that make only sperm at 25C is no different from that of wild-type young adults (Fig. 4B), indicating that sperm also contains clk-2 mRNA."
"Although all FEM-2/FEM-3 pairs from a single species interact, only two out of six interspecific pairs bind each other, showing that FEM-2 and FEM-3 are coevolving. Both interspecific interactions involved C. briggsae FEM-3."
FEM-3 is absent from the distal wild-type adult gremlin detected by anti- FEM-3 antibodies. In nos-3(0), FEM-3 accumulates throughout the distal germline, supporting a role for NOS-3-mediated repression of fem-3 translation in this region.
"When expressed in mammalian cells, SEL-10 protein coimmunoprecipitates with FEM-1, FEM-2, and FEM-3, which are required for masculinization, and FEM-1 and FEM-3 are targeted by SEL-10 for proteasomal degradation."
"When expressed in mammalian cells, SEL-10 protein coimmunoprecipitates with FEM-1, FEM-2, and FEM-3, which are required for masculinization, and FEM-1 and FEM-3 are targeted by SEL-10 for proteasomal degradation."