The unc-37(e262) mutation does not prevent the expression of an unc-4lacZ transgene in the VA motor neurons. In addition the steady state level of mRNA, transcribed from the endogenous unc-4 gene in unc-37(e262) is comparable to wild type during the developmental period in which the VA motor neurons accept synaptic input from interneurons.
The percentage of aggregates increased compared to animals overexpressing the transgene alone. Animals exhibited reduced mobility like that of rmIs263 alone. This locomotion defect improves with the treatment with 17-DMAG. Further, valproic acid (VA) did not rescue the motor dysfunction exhibited by these animals.
During the mid-L2 larval stage, del-1::GFP expression in the ventral nerve cord is largely restricted to the VB class of motor neurons. In the unc-37 mutant, del-1::GFP is also expressed ectopically in the VA motor neurons in mid-L2 larvae.
During the mid-L2 larval stage, del-1::GFP expression in the ventral nerve cord is largely restricted to the VB class of motor neurons. In the unc-4 null alleles wd1 and e120, del-1::GFP is also expressed ectopically in the VA motor neurons in mid-L2 larvae.