"Northern blots of
unc-1(0) and
unc-24(0) animals, probed with an
unc-1 cDNA, show that the
unc-1 gene was transcribed into a 1.2-kb message in N2 and
unc-24 animals but not in
unc-1(0) animals (Fig. 5A). Probing with the actin gene
act-1 showed equal loading in each lane (data not shown). We then probed Western blots of the same strains with the anti-UNC-1 antibody. These blots showed decreased staining of the predominant protein in both
unc-1 and
unc-24 animals compared with N2 (Fig. 5B)."