In vitro translated, full-length TRA-1A protein could bind to and shift a probe consisting of a 297 bp DNA fragment that we amplified from sequences 5526 to 5823 bp downstream of the
egl-1 stop codon. TRA-1A was able to bind to the probe containing the putative TRA-1A-binding site amplified from wild-type animals but failed to bind to the corresponding probes amplified from animals carrying the
egl-1(gf) mutation
n1084,
n2164, or
n2248, which represent the three differ- ent G-to-A transitions.