"As mentioned above, DAF-16 activity is primarily regulated by its subcellular localization (Alam et al., 2010). We hence used the
daf-16::GFP reporter strain to establish DAF-16 localization at day 3 following AZD2281, NR, or NAM exposure. In line with the increased
sod-3 transcriptional activation (Figure 4C),
daf-16 appeared in a more punctate pattern consistent with its nuclear localization that is required for it to activate transcription of its antioxidant target genes (Figures 4G and S5B). Quantitative analysis of this daf- 16 translocation after these various stimuli shows that approximately two to three times more
daf-16 is localized in the nucleus (Figures 4H and S5C)."