Monomeric red fluorescent protein (tagRFP) tagged KIN-19 (KIN-19::tagRFP) is expressed abundantly in the worm pharynx. KIN-19::tagRFP expressed in the anterior pharynx undergoes spontaneous age-dependent aggregation. KIN-19 aggregates increased ~5-fold when worms are exposed to 400 mM NaCl for 24 h.
"HA-DAF-18 was pulled down by using a PDZ domain-containing KIN-4 C-terminal fragment [C],but not by an N-terminal KIN-4 fragment [N] or by a kinase domain-containing KIN-4 middle fragment [Mid]."; "GST KIN-4 PDZ strongly bound to FLAG-DAF-18 WT [WT] but weakly to FLAG-DAF-18 delta4C and FLAG-DAF-18 delta12C."
"We detected several known midbody components (ZEN-4, CYK-4, ICP-1 and PLK-1) in KIN-3::GFP coprecipitates, suggesting a role of KIN-3 as part of the midbody structure."
"We detected several known midbody components (ZEN-4, CYK-4, ICP-1 and PLK-1) in KIN-3::GFP coprecipitates, suggesting a role of KIN-3 as part of the midbody structure."