"In embryos depleted of Ran or any of its cofactors, RanBP2, RanGAP, or RCC1, no NE-like GFP::EMR-1 signal was detected after the first cell division in the majority of cases (67%) (Figure 8, B and D-F, Video 13). In other embryos, presumably where the RNAi had been less efficient, aberrant nuclei with a strong aggregation of GFP::EMR-1 next to the NE became apparent after a prolonged time (Figure 8C)... Embryos depleted of IMB-1 showed a strong defect in GFP::EMR-1 recruitment to the NE-like structures after mitosis (Figure 7H and Video16, 3 of 3 embryos). As with embryos where Ran or its cofactors had been targeted,
imb-1(RNAi) embryos instead showed clustering of GFP::EMR-1 at the centrosomes."