In wild-type animals, after 2 to 3 hours, the lag-2
::GFP signal increased in the DTCs (Z1.aa/Z4.pp) and decreased in their sister cells (Z1.ap/Z4.pa). After about 5 hours, when the DTCs started to migrate, lag-2
::GFP was expressed nearly exclusively in the DTCs and not in their sisters. The extra lag-2
::GFP-positive cells in cdk-2
(RNAi) animals were always observed at the position of Z1.ap/Z4.pa at the end of the L1 stage, and these cells migrated distally without further divisions, indicating that the Z1.ap/Z4.pa cells had transformed into DTCs.