Northern blot analysis of total RNA from wild-type (N2) or
vab-3(
e648) mixed stage worm populations showed that the
ceh-32 mRNA levels are reduced by a factor of 5 in
vab-3(
e648) compared to wild type. CEH-32 expression in the SS precursor cells at the L2/L3 stage, later in gonadal sheath cells, and in neuronal and hypodermal head cells. However, in some
vab-3(
e648) worms, CEH-32 expression was not detected in some head hypodermal cells normally expressing
ceh-32.