"mutant pi cells properly establish expression of two pi-specific markers, lin-11::gfp and cog-2::gfp... ku194 animals carrying the lin-11::gfp integrated array express LIN-11::GFP in a similar pattern, indicating that the pi cell fate is intact with regard to expression of this lineage marker (Fig. 5C,D)."
lin-12 is activated in the presumptive pi and rho cells, leading to rho cells being converted into pi cells independent of the AC and LAG-2 signal. The nhr-67(pf2); lin-12(n137gf) strain that carries an egl-13::gfp reporter was strongly suppressed for the lin-12(gf)616 effect on pi cells.
Uterine lin-11 fluorescence was not detected at pi cell specification or later relevant stages in fos-1 mutants. Conversely, appropriate lin-11 expression was observed in the 2 vulval lineage in fos-1 mutants as in the wild type.
Uterine lin-11 fluorescence was not detected at pi cell specification or later relevant stages in fos-1 mutants. Conversely, appropriate lin-11 expression was observed in the 2 vulval lineage in fos-1 mutants as in the wild type.
In comparison to control RNAi animals, the RNAi-mediated knock-downs of lag-1 led to significant reduction in the number of animals expressing Cel-lin-11p::GFP in pi progeny. GFP fluorescence in vulval cells was reduced in lag-1 RNAi animals.
In comparison to control RNAi animals, the RNAi-mediated knock-downs of egl-43 led to significant reduction in the number of animals expressing Cel-lin-11p::GFP in pi progeny. GFP fluorescence in vulval cells was completely absent in egl-43 RNAi animals.
In comparison to control RNAi animals, the RNAi-mediated knock-downs of fos-1 led to significant reduction in the number of animals expressing Cel-lin-11p::GFP in pi progeny. GFP fluorescence in vulval cells was completely absent in fos-1 RNAi animals.
an average of 4.2 of 6 pi cells per animal-side showed lin-11::lacZ beta-galactosidase staining. By contrast, analysis of 22 sel-12(ty11) and 27 sel-12(ar171) mutant animals revealed an average of 0.95 and 0.37 staining cells per animal-side, respectively.