In wild-type embryos, PAR-3 is localized to the anterior periphery of a one-cell embryo; after the first division, PAR-3 surrounds the ABblastomere, but is present only at the anterior periphery of P1. In
par-6 mutants PAR-3 peripheral staining is reduced or absent. In addition, when staining is detectable, it is not always asymmetric.