Cortical Z-series projections revealed that in wild- type or control embryos expressing GFP-PAR-2, PAR-2 is recruited to the posterior cortex as the posterior end of the embryo begins smoothing. On average, 43.8%4.28% (n=5 embryos) of the cortical area is occupied by GFP-PAR- 2 by pseudocleavage formation. This area decreases slightly as the embryo progresses through maintenance phase, occupying 39.78%7.57% (n=5 embryos) of the total cortical area at pronuclear meeting and remaining steady through 4:00 min after pronuclear meeting. In
arx-2 RNAi- treated embryos, the area occupied by GFP-PAR-2 appears unaffected during establishment phase, however, PAR-2 expands beyond the typical domain during maintenance phase to occupy almost 60% of the total cortical area over the course of multiple time points.