In control embryos, GFP::DHC-1 localized to the nuclear periphery in prophase and was associated with chromosomes upon nuclear envelope breakdown. At metaphase, kinetochore and K-Mt localization was evident. At anaphase, GFP::DHC-1 was no longer detectable at kinetochores but was still apparent on K-Mts and centrosomes. In
cyb-3(RNAi) embryos, GFP::DHC-1 localized to the nuclear periphery and centrosomes during prophase and also accumulated at kinetochores during prometaphase and metaphase as in controls. Strikingly, there was little or no apparent localization to K-Mts or centrosomes at anytime after nuclear envelop breaking.