We followed single
mab-3;
dmd-3 mutant males throughout their L4 stage to ensure that we were not missing the window of expression because
mab-3;
dmd-3 mutants do not undergo retraction and the exact stage can be difficult to determine. Similar to wild-type L4 hermaphrodites,
mab-3;
dmd-3 mutants failed to show
mtre-1::GFP expression at any point during the L4 stage.