The TGTTTGT sequence was mutated to GTGGGTG, resulting in pTMmutPHA-4. Transgenic worms con- taining pTMmutPHA-4 failed to express the GFP in either pharynx or intestine.
When the CdxA homolog site was mutated in pTMmutCdxA, reporter expression was not observed in either the pharynx or the intestine throughout development.