36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. TSN-1 is a homolog of micrococcal nuclease that was shown to be associated with RISC and required for
let-7 miRNA function (Caudy et al., 2003), which is consistent with the involvement of AIN-2 in the miRNA effector complex. GLD-1 and ACO-1, two RNA-binding proteins involved in translational inhibition (Gourley et al., 2003; Jan et al., 1999; Lee and Schedl, 2001), were also found to be in the AIN-2 IP sample, suggesting that miRNA may collaborate with RNA-binding proteins to repress the translation of target mRNAs.