"To identify an optimal time for collection of RNA after heat shock, we examined the induction of the Hox gene
lin-39, a putative Wnt target in the VPCs (Eisenmann et al. 1998; Wagmaister et al. 2006), and the LIN-39 target
egl-18, which is likely to be an indirect Wnt pathway target in those cells (Koh et al. 2002). Based on these data (Figure 1C), and to bias the microarray analysis toward possible direct Wnt pathway target genes, we allowed heat-shocked animals to recover for only 60 min before collecting RNA."