These animals showed no hlh-8::gfp expression throughout the M lineage and lacked all M-derived CCs. This is an enhancement of the defects in the M-lineage compared to either RNAi or mutant alone.
These animals showed no hlh-8::gfp expression throughout the M lineage and lacked all M-derived CCs. This is an enhancement of the defects in the M-lineage compared to either RNAi or mutant alone.
36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. Among them, we found many components of the core translational initiation complex, including PAB-1 and PAB-2 (the two C. elegans cytoplasmic polyadenylate-binding proteins, or PABPC [Mangus et al., 2003]), IFG-1 (the C. elegans ortholog of eIF4G [Dinkova et al., 2005]), and RPS-0 (a component of the 40S ribosome subunit [Ford et al., 1999]).