In non-molting animals,
unc-95::gfp shows the known pattern of DB, which is characterized by equally spaced spots about 0.6 microns in diameter laying along imaginary straight lines. Fluorescence intensity profiles drawn along such lines show very low diffusive levels and narrow peaks across each DB. UNC-95-GFP showed a 36% decrease in average intensity at the DB during molting. Western blot analysis using anti-GFP antibodies indicated there were no changes in total protein levels of UNC-95:GFP during molting, suggesting the observed reduction in intensity is due to changes in localization.