"When wild-type animals expressing the
lag-2::GFP transgene were grown 12 h on HU beginning at the L2, or L3 stage, and subsequently removed to plates without the drug and allowed to develop to adulthood, two DTCs were formed, although all stage-specific cell divisions were blocked by HU. Therefore, this HU treatment has no effect on the production or maintenance of this cell type. However, when early L2- or L3-stage
cki-1(RNAi) larvae were treated with HU, extra DTCs were still generated (Table 4)."