In 58% of the
hlh-2 RNAi-treated animals (n=86), the gfp was expressed in the vms, compared to 92% of the control animals (n = 92). The hmc and enteric muscles are already born and expressing the gfp at the time of treatment so this experiment would only detect whether CeE/DA was required for maintenance rather than initial expression in these cells. The hmc expression decreased from 91% in control animals to 84% in treated animals and expression in the enteric muscles decreased from 100% in control animals to 93% in treated animals.