"The expression of HIF-1:GFP was assayed by immunoblot by using a GFP-specific antibody. As shown in Fig. 3, HIF-1:GFP expression is increased by hypoxia treatment, and the protein is rapidly degraded upon reoxygenation.
hif-1 mRNA is not substantially increased by hypoxia treatment (data not shown). The levels of AHA-1 protein remained constant, regardless of oxygen concentration (Fig. 3)."