HIF-1 protein levels increase during exposure to H2S. As with hypoxia, H2S exposure results in increased protein concentration in as little as 45 minutes. Immunostaining reveals HIF-1 accumulation in the nuclei throughout the H2S treated worms, as compared to untreated controls.
sqrd-1 and cysl-2 mRNA levels increase during RA/H2S and RA/HCN exposure. SQRD-1 protein levels increase during RA/H2S exposure in the parental N2 Bristol strain.