eif-6(RNAi) results in an approximately twofold accumulation of LIN-14 at the 20 h time point, relative to the vector (RNAi) control, and this misregulation continues to be apparent up to 30 h of development.
Early in the L1 (at 5 h of development), the number and intensity of anti-LIN-14 fluorescent nuclei were greatly reduced in lin-4-overexpressing animals compared to similarly staged wild-type animals.
The lin-14(n355) mutation (a gain-of-function which removes lin-4 binding sites from the lin-14 3'UTR) partially suppresses the eif-6(RNAi)-induced accumulation of LIN-14 protein