In wild-type, GFP:MEI-1 levels remain steady for w25 30 min after egg exit from the spermatheca. In contrast, in egg-3(tm1191) mutants, GFP:MEI-1 levels started to decline immediately after spermatheca exit, reaching background levels by 30 min.
The recruitment of RAB-7 onto the phagosome was delayed from 70 15 min in WT(n=11) to 102 30 min in epg-5 (n = 12) mutants. The GFP::RAB-7 ring was less stable in the epg-5 mutant than that of WT.
"Nuclear localization induced by starvation is rapidly reversed by exposure to food (E. coli). When starved animals with nuclear localized DAF-16::GFP are placed on an NGM plate seeded with E. coi, delocalization is first evident at 5 min and is complete in 10 min (see Figure 3b4)."
When we examined the effect of removing lin-1 activity on the expression of the full-length reporter arIs131[lag-2p::2Xnls::yfp] or the lag-2p(min) reporter arEx1098[lag-2p(min):: 2nls-yfp], we observed strong and uniform derepression of lag-2 in all six VPCs in lin-1(0) mutants.
Reduction of odr-10 levels by RNAi increased daf-2 motility on bacteria significantly, suppressing the decreased velocity seen on bacteria in the daf-2(e1370) mutant alone. daf-2 mutants will stop moving after 1.5 to 3 min when placed onto bacteria, but with reduction of odr-10 levels by RNAi, worms continue to move constantly over a 10 min assay period.