"C. elegans PAR-1 binds to NMY-2, a conventional nonmuscle myosin (Guo and Kemphues, 1996). To test whether PAR-1 played a role in cytoskeletal organization in vulval cells, we examined the distribution of NMY-2 and tubulin. At the resolution of the light microscope in fixed specimens, we found no obvious differences in the expression or organization of these components of the cytoskeleton after depletion of PAR-1 [Fig. 5, and unpublished results; a minimum of 5 control and 5
par-1(RNAi) larvae were examined for each]."