"To validate the microarray experiments, we measured transcript levels for ten selected 'biomarker' genes by qRT-PCR over a time course of infection... All ten genes tested were induced in response to infection (Figure 4A, B). A subset of these biomarkers,
fmo-2 (FMO),
ilys-3 (lysozyme),
cpr-2 (protease), Y65B4BR.1 (lipase),
exc-5 (GEF), and F53A9.8 (putative antimicrobial peptide), were already induced 10-fold or higher by the first time-point at 4 h (Figure 4A). A second subset,
lys-5 (lysozyme),
clec-52,
clec-60, and
clec-71 (CTLs), were only modestly induced by 4 h and exhibited a further increase by 12 h (Figure 4B). Thus, time-resolved gene expression analysis revealed the existence of at least two kinetic groups, defined by their expression levels at 4 h."