"Representative transcript genes from this dataset, along with selected sulfur metabolism related genes, were analyzed by qRT-PCR of total RNA from three independent cultures grown in each Se concentration (Table 4, Fig. S3-S5). For the seven oxidoreductases listed in Table 4, the fold changes determined by qRT-PCR analysis ranged from 1.32 to 4.70 at 0.2 mM Se, and 1.85 to 4.41 at 0.4 mM Se, thus confirming the microarray analysis. qRT-PCR analysis similarly confirmed that five sulfur metabolism-related genes, changed >2-fold as assessed by microarray analysis, were up-regulated by toxic Se (Fig. S3, S4A-S4D); this includes
gst-19 (in the glutathione-s-transferase family) and
cpr-2 (putative cysteine-type peptidase (Fig. S5F), which were elevated .35 fold by 0.2 or 0.4 mM Se treatment as assessed by either microarray or qRT-PCR. Similar up-regulation was found for two ER stress-related genes which were elevated . 3-fold by 0.4 mM Se, as assessed by qRT-PCR. Additionally, two transporter gene transcripts were also up-regulated by toxic Se treatment as assessed by qRT-PCR (Fig. S3E-S3H). Lastly, qRT-PCR analysis of an additional five sulfur metabolism transcripts, which by microarray analysis were regulated ,1.4- fold by 0.2 and 0.4 mM Se (including
trxr-1,
trxr-2, and
glrx-21), confirmed the limited modulation of these transcripts by Se status (Table 4, Fig. S4E-S4H)."