"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to
act-1 levels after
sams-1(RNAi) (not shown), as well as in
sams-1(
ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the
act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."