In WT worms, ARL-13 is found exclusively in cilia. However, in IFT-B, IFT-A, IFT-dynein and BBS gene mutants, ARL-13 was specifically mislocalised at the periciliary membrane (PCM) region and not in other regions of the sensory neuron.
When introduced into a dyf-2 mutant background, OSM-5::GFP was no longer located at the base of cilia and was diffusely spread throughout the dendrites, indicating that DYF-2 is required for ciliary loading of OSM-5 molecules.