"In addition to extra DTCs, frequently
cki-1(RNAi)-affected animals had one or no DTCs based on the absence of
lag-2::GFP expression in the gonad arms. The DTC-lacking gonad arms of these animals showed no elongation or mitotic proliferation of the germ cell nuclei, suggesting that the absence of
lag-2::GFP expression in these cells faithfully reflects the loss of DTC identity (Fig. 1D)."