"We evaluated this question by crossing the grk-2
) and grk-2
) alleles with strains that are constitutive egg layers. These strains include a gain-of-function strain that has activated Gαq (egl-30
)) as well as loss-of-function strains that are defective in Gαo (goa-1
)), the G protein that inhibits egg laying; EAT-16 (eat-16
)), the regulator of G protein signaling (RGS) protein of Gαq; or diacylglycerol kinase (dgk-1
)), a potential effector enzyme that phosphorylates diacylglycerol. Interestingly, the constitutive egg laying phenotype in these strains could be effectively suppressed by grk-2
(lf) (Fig. 3A), suggesting that GRK-2 may function downstream of these proteins in the egg laying pathway."