Authors mutated AATGTCAT at -396 to AACTGCAG in the Pnlg-1::gfp reporter to create a reporter with a deleted binding site (the Pnlg-1(Dbs)::gfp reporter; Figure 4A). Basal motor neuron fluorescence of transgenic animals expressing Pnlg-1(Dbs)::gfp was similar to transgenic animals expressing the Pnlg-1::gfp reporter (Figure 4B). However, Pnlg-1(Dbs)::gfp reporter fluorescence did not increase in either
wdr-23 mutants or in
skn-1(gf) mutants compared to wild type controls (Figure 4B, 4C and 4F), suggesting that this site is critical for
skn-1-mediated increases in
nlg-1 expression.