lbp-8 mRNA amounts were increased in lipl4 Tg compared with WT but not in the transgenic strain overexpressing lipl-4 that lacks the signal peptide for lysosomal expression (lipl-4 Tg no SP).
Concordantly, the longevity extensions by lipl-4 Tg and eat-2(ad1116), a genetic model of dietary restriction in C. elegans (13), were additive (fig. S10).
nhr-49 RNAi reduced the life span of long-lived lbp-8-overexpressing transgenic (Tg) worms to that of short-lived nhr-49(RNAi) worms, suggesting that nhr-49(RNAi) is epistatic to lbp-8 overexpression
nhr-49 RNAi reduced the life span of long-lived lipl-4-overexpressing transgenic (Tg) worms to that of short-lived nhr-49(RNAi) worms, suggesting that nhr-49(RNAi) is epistatic to lipl-4 overexpression