In hermaphrodite smIs54 embryos, CEH-30 GFP was never seen in ventral CEMs. In contrast, in
tra-1(
n1099); smIs54 XX embryos, which are completely masculinized and virtually identical to male embryos, CEH-30 GFP was observed in all ventral CEMs, suggesting that TRA-1 directly suppresses CEH-30 expression in hermaphrodite CEMs.