lon/C34B2.6 RNAi resulted in increased expression of the
atfs-1p::
atfs-1::GFP translational fusion reporter, suggesting that the Lon protease encoded by C34B2.6 actively degrades the ATFS-1 transcription factor protein. lon/C34B2.6 RNAi also resulted in increased expression of endogenous ATFS-1 protein, as determined by Western blot. Cell fractionation after lon/C34B2.6 RNAi suggests that ATFS-1 accumulated in the mitochondrial matrix